COX4I1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COX4I1 full-length
Host
Human
Theoretical MW (kDa)
19.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COX4I1 antibody (H00001327-D01P) by Western Blots.
SDS-PAGE Gel
COX4I1 transfected lysate.
Western Blot
Lane 1: COX4I1 transfected lysate ( 19.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COX4I1
Entrez GeneID
1327GeneBank Accession#
NM_001861.2Protein Accession#
NP_001852.1Gene Name
COX4I1
Gene Alias
COX4, COXIV, MGC72016
Gene Description
cytochrome c oxidase subunit IV isoform 1
Omim ID
123864Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq
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