BDNF monoclonal antibody (M02), clone 1B10
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a full-length recombinant BDNF.
Immunogen
BDNF (AAH29795, 1 a.a. ~ 247 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MTILFLTMVISYFGCMKAAPMKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHMIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYKTKCNPMGYAKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.91 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
BDNF monoclonal antibody (M02), clone 1B10. Western Blot analysis of BDNF expression in IMR-32.Western Blot (Transfected lysate)
Western Blot analysis of BDNF expression in transfected 293T cell line by BDNF monoclonal antibody (M02), clone 1B10.
Lane 1: BDNF transfected lysate(28.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of BDNF transfected lysate using anti-BDNF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BDNF monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BDNF is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to BDNF on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — BDNF
Entrez GeneID
627GeneBank Accession#
BC029795Protein Accession#
AAH29795Gene Name
BDNF
Gene Alias
MGC34632
Gene Description
brain-derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
Other Designations
neurotrophin
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Interactomes
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Pathways
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Diseases
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