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BIOtech
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APRT 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000353-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-APRT full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 19.91
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-APRT antibody (H00000353-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000353-T01
    APRT transfected lysate.
    Western Blot
    QC Testing of H00000353-T01
    Lane 1: APRT transfected lysate ( 19.91 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 353
  • Gene Name:
  • APRT
  • Gene Alias:
  • AMP,DKFZp686D13177,MGC125856,MGC125857,MGC129961
  • Gene Description:
  • adenine phosphoribosyltransferase
  • Gene Summary:
  • Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • AMP diphosphorylase,AMP pyrophosphorylase,adenine phosphoribosyltransferase, isoform a,transphosphoribosidase
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