We provide a fast and reliable scale-up monoclonal antibody from transient production. Utilizing a dual high-copy vector system, F(ab)2 and Fc fragments are co-transfected into a mammalian cell line. These transfected cells are then amplified in optimized serum-free, suspension culture, and the expressed antibodies are purified with affinity chromatography. Final products are quality-tested with IgG antibody SDS-PAGE Coomassie Blue staining and ELISA. Transient antibody production has the ability to produce the desired quantity of up to 100mg within 2-3 weeks.

Custom_Transient_Antibody_Production.pdf

Transient Antibody Production Workflow
  • Plasmid Vector :
    pAB03(with Fc) + Fd
    pAB04 + LC
  • Host Cell :
    293H
    293F
    293T
  • QC Data
 
 

Advantages

Applications
  • High Affinity Antibody
  • Diverse Antibody Repertoire
  • No Immunologic Tolerance
  • Scalable Antibody Production
  • More Stable Antibody Clone
  • Antibody Engineering
  • High Affinity Antibody
  • Rabbit Monoclonal Antibody
  • Modification-State Antibody
  • Anti-Idiotype Antibody
  • Antibody Pairing and ELISA Kit
  • Research and In Vitro Diagnostic
 
 

Example
Antibody Concentration versus Yield
  Production Site Host Cell Antibody Concentration (μg/uL) Yield
(mAb μg/Culture Sup. mL)
Humanized CD3 monoclonal antibody Other Company 293F 1.08 10
Abnova 293H 4.84 70
293F 1.52 30
 
 

Flow Cytometry


Flow cytometry of human CD3 T cells stained humanized CD3 monoclonal Ab (1 µg/5*105 cells) and counterstained with PE anti-human IgG Ab (1 µg/5*105 cells).
 

For any inquiry, please contact : OEM@abnova.com

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