Abnova offers high quality, efficient and cost-saving conjugation service between protein and fluorescent dyes, phycobiliproteins, streptavidin, biotin and enzymes. Since every protein has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total protein of interest will be tested and analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the protein sample.

Custom_Protein_Conjugation.pdf

General Working Progress of Protein Conjugation
  • Evaluation and Discussion of Conjugation Requirement and Service
  • Completion of the Custom Conjugation Specification (,)
  • Confirmation of Receipt of Antibody/Protein Sample
  • Conjugation Buffer Exchange
  • Antibody/Protein Concentration and Volume Analysis
  • Test-Scale Conjugation Optimization
  • Mass Antibody/Protein Labeling
  • Chromatographic Gel Purification
  • Spectrophotometer Measurement
  • COA, Packing, and Shipment
 
 

Table of Labeling Molecules and Excitation/Emission Wavelengths
Labeling Molecule Excitation Max (nm) Emission Max (nm)
Biotin - -
HRP - -
Alexa 488 490 525
FITC 490 525
PE 535 575
TRITC 546 575
Cy3 554 568
Alexa 555 555 580
Alexa 594 590 617
Texas Red 595 616
APC 650 660
Alexa 647 650 665
Cy5 650 670
DyLight 650 652 672
 
 

Special Notes:
  1. The protein of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of protein from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.
  2. Minimum quanity of antibody conjugation is 0.2mg. Minimum concentration is 0.1mg/ml.
 
 

Example
QC Data of Fluorochrome-Labeled Protein
 

 
The PC9 were detected by FITC conjugated PanCK monoclonal antibody (green), white blood cells were detected by PE conjugated CD45 monoclonal antibody (orange) and nucleus were detected by DAPI counter stain (blue).
 

For any inquiry, please contact : OEM@abnova.com

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