Abnova offers high-quality, efficient, and cost-saving conjugation service bridging antibody and fluorescent dyes, phycobiliproteins, streptavidin, biotin, and enzymes. Since every antibody has its unique characteristics, conjugation needs to be optimized. 0.2mg of total antibody of interest will be tested and analyzed prior to mass labeling. The tested sample will be mixed with the mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the antibody sample.

Custom_Antibody_Conjugation.pdf

General Working Progress of Antibody Conjugation
  • Evaluation and Discussion of Conjugation Requirement and Service
  • Completion of the Custom Conjugation Specification ( , )
  • Confirmation of Receipt of Antibody/Protein Sample
  • Conjugation Buffer Exchange
  • Antibody/Protein Concentration and Volume Analysis
  • Test-Scale Conjugation Optimization
  • Mass Antibody/Protein Labeling
  • Chromatographic Gel Purification
  • Spectrophotometer Measurement
  • COA, Packing, and Shipment
 
 

Table of Labeling Molecules and Excitation/Emission Wavelengths

Labeling Molecule

Excitation Max (nm)

Emission Max (nm)

Emission Spectrum (nm)

iFluor 350

345

450

  

FITC

497

516

  

iFluor 488

491

516

  

Cy3

555

569

  

iFluor 555

557

570

  

iFluor 594

587

603

  

PE-Texas Red

565

613

  

iFluor 633

640

654

  

APC

651

660

  

PE-Cy5

565

666

  

Cy5

651

670

  

iFluor 647

656

670

  

PE-Cy5.5

565

671

  

PerCP

477

678

  

APC-Cy5.5

651

700

  

iFluor 680

684

701

  

iFluor 700

690

713

  

PE-Cy7

565

778

  

APC-Cy7

651

779

  

ALP

N/A

N/A

N/A

Biotin

N/A

N/A

N/A

HRP

N/A

N/A

N/A

Special Notes
  1. The antibody of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of antibody from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.

  2. Minimum quantity of antibody conjugation is 0.2mg. The minimum concentration is 0.1mg/ml.
 
 

Examples

Immunofluorescence

Service_Antibody-Conjugation_IF.jpg (26 KB)

The PC9 was detected by FITC conjugated PanCK monoclonal antibody (green),  white blood cells were detected by PE-conjugated CD45 monoclonal antibody (orange) and the nucleus was detected by DAPI counterstain (blue).

 

Flow Cytometry

 Service_Antibody-Conjugation_flow.jpg (18 KB)

Flow cytometry analysis of CD8A on enriched human CD8 T cell from a healthy person. CD8 T cells were treated with CD8A VHH humanized monoclonal antibody, clone B5 conjugated with DyLight 650.
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