Abnova offers high quality, efficient and cost-saving conjugation service between antibody and fluorescent dyes, phycobiliproteins, streptavidin, biotin and enzymes. Since every antibody has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total antibody of interest will be tested and analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the antibody sample.

Custom_Antibody_Conjugation.pdf

General Working Progress of Antibody Conjugation
  • Evaluation and Discussion of Conjugation Requirement and Service
  • Completion of the Custom Conjugation Specification ( , )
  • Confirmation of Receipt of Antibody/Protein Sample
  • Conjugation Buffer Exchange
  • Antibody/Protein Concentration and Volume Analysis
  • Test-Scale Conjugation Optimization
  • Mass Antibody/Protein Labeling
  • Chromatographic Gel Purification
  • Spectrophotometer Measurement
  • COA, Packing, and Shipment
 
 

Table of Labeling Molecules and Excitation/Emission Wavelengths

Labeling Molecule

Excitation Max (nm)

Emission Max (nm)

Biotin

 - -

HRP

 - -

Alexa 488

 490 525

FITC

 490 525

PE

 535 575

TRITC

 546 575

Cy3

 554 568

Alexa 555

 555 580

Alexa 594

 590 617

Texas Red

 595 616

APC

 650 660

Alexa 647

 650 665

Cy5

 650 670

DyLight 650

 652 672

Special Notes
  1. The antibody of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of antibody from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.
  2. Minimum quanity of antibody conjugation is 0.2mg. Minimum concentration is 0.1mg/ml.
 
 

Examples

For any inquiry, please contact : OEM@abnova.com

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