Abnova offers high quality, efficient and cost-saving conjugation service between antibody and fluorescent dyes, phycobiliproteins, streptavidin, biotin and enzymes. Since every antibody has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total antibody of interest will be tested and analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the antibody sample.

General Working Progress of Antibody Conjugation

  • Evaluation and Discussion of Conjugation Requirement and Service
  • Completion of the Custom Conjugation Specification ( , )
  • Confirmation of Receipt of Antibody/Protein Sample
  • Conjugation Buffer Exchange
  • Antibody/Protein Concentration and Volume Analysis
  • Test-Scale Conjugation Optimization
  • Mass Antibody/Protein Labeling
  • Chromatographic Gel Purification
  • Spectrophotometer Measurement
  • COA, Packing, and Shipment

Table of Labeling Molecules and Excitation/Emission Wavelengths

Labeling Molecule Excitation Max (nm) Emission Max (nm)
Biotin - -
HRP - -
Alexa 488 490 525
FITC 490 525
PE 535 575
TRITC 546 575
Cy3 554 568
Alexa 555 555 580
Alexa 594 590 617
Texas Red 595 616
APC 650 660
Alexa 647 650 665
Cy5 650 670
DyLight 650 652 672

Special Notes:

  1. The antibody of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of antibody from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.
  2. Minimum quanity of antibody conjugation is 0.2mg. Minimum concentration is 0.1mg/ml.


For any inquiry, please contact : OEM@abnova.com