PDGFRB (phospho Y740) recombinant monoclonal antibody, clone 3B8
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More Files
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Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (not treated), Lane 2: Hela whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 3: Hela whole cell lysate (treated with Pervanadate), Lane 4: A549 whole cell lysate (not treated) and Lane 5: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) with PDGFRB (phospho Y740) recombinant monoclonal antibody, clone 3B8 (Cat # RAB04255).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PDGFRB.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y740 of human PDGFRB.
Theoretical MW (kDa)
Calculated MW: 190 k
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Hela whole cell lysate (not treated), Lane 2: Hela whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 3: Hela whole cell lysate (treated with Pervanadate), Lane 4: A549 whole cell lysate (not treated) and Lane 5: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) with PDGFRB (phospho Y740) recombinant monoclonal antibody, clone 3B8 (Cat # RAB04255).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDGFRB
Entrez GeneID
5159Protein Accession#
P09619Gene Name
PDGFRB
Gene Alias
CD140B, JTK12, PDGF-R-beta, PDGFR, PDGFR1
Gene Description
platelet-derived growth factor receptor, beta polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq
Other Designations
beta platelet-derived growth factor receptor|platelet-derived growth factor receptor beta|soluble PDGFRb variant 1
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Interactome
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Pathway
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Disease
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