STAT5A (phospho Y694) recombinant monoclonal antibody, clone 3F1
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More Files
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Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 2: HepG2 whole cell lysate (not treated) with STAT5A (phospho Y694) recombinant monoclonal antibody, clone 3F1 (Cat # RAB04241).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with STAT5A (phospho Y694) recombinant monoclonal antibody, clone 3F1 (Cat # RAB04241) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human STAT5A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Y694 of human STAT5A.
Theoretical MW (kDa)
Calculated MW: 90 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 2: HepG2 whole cell lysate (not treated) with STAT5A (phospho Y694) recombinant monoclonal antibody, clone 3F1 (Cat # RAB04241).Immunohistochemistry
Immunohistochemical staining of human breast cancer with STAT5A (phospho Y694) recombinant monoclonal antibody, clone 3F1 (Cat # RAB04241) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — STAT5A
Entrez GeneID
6776Protein Accession#
P42229Gene Name
STAT5A
Gene Alias
MGF, STAT5
Gene Description
signal transducer and activator of transcription 5A
Omim ID
601511Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq
Other Designations
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