PAK1/PAK2/PAK3 (phospho S144/S141/S139) recombinant monoclonal antibody, clone 3H12
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More Files
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Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 2: A549 whole cell lysate (not treated) with PAK1/PAK2/PAK3 (phospho S144/S141/S139) recombinant monoclonal antibody, clone 3H12 (Cat # RAB04240).
Immunohistochemistry
Immunohistochemical staining of rat brain tissue with PAK1/PAK2/PAK3 (phospho S144/S141/S139) recombinant monoclonal antibody, clone 3H12 (Cat # RAB04240) (diluated at 1:100).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human PAK3/PAK1/PAK2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S144/S141/S139 of human PAK1/PAK2/PAK3.
Theoretical MW (kDa)
Calculated MW: 65 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: A549 whole cell lysate (treated with EGF 100 ng/mL/20 mins) and Lane 2: A549 whole cell lysate (not treated) with PAK1/PAK2/PAK3 (phospho S144/S141/S139) recombinant monoclonal antibody, clone 3H12 (Cat # RAB04240).Immunohistochemistry
Immunohistochemical staining of rat brain tissue with PAK1/PAK2/PAK3 (phospho S144/S141/S139) recombinant monoclonal antibody, clone 3H12 (Cat # RAB04240) (diluated at 1:100).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PAK1
Entrez GeneID
5058Protein Accession#
O75914;Q13153;Q13177Gene Name
PAK1
Gene Alias
MGC130000, MGC130001, PAKalpha
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 1
Omim ID
602590Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
STE20 homolog, yeast|p21-activated kinase 1|p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)|p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
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Gene Info — PAK2
Entrez GeneID
5062Protein Accession#
O75914;Q13153;Q13177Gene Name
PAK2
Gene Alias
PAK65, PAKgamma
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 2
Omim ID
605022Gene Ontology
HyperlinkGene Summary
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq
Other Designations
S6/H4 kinase|p21 (CDKN1A)-activated kinase 2|p21-activated kinase 2
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Gene Info — PAK3
Entrez GeneID
5063Protein Accession#
O75914;Q13153;Q13177Gene Name
PAK3
Gene Alias
CDKN1A, MRX30, MRX47, OPHN3, PAK3beta, bPAK, hPAK3
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 3
Gene Ontology
HyperlinkGene Summary
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000023855|OTTHUMP00000062894|beta-PAK|oligophrenin-3|p21 (CDKN1A)-activated kinase 3|p21-activated kinase 3|p21-activated kinase-3|serine/threonine-protein kinase PAK 3
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Interactome
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Pathway
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Disease
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