CD9 recombinant monoclonal antibody, clone 3A2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of U87 whole cell lysate with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182).
Immunohistochemistry
Immunohistochemical staining of human small intestine tissue with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182) (diluated at 1:34). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD9.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CD9.
Theoretical MW (kDa)
Calculated MW: 25 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:30-1:200)
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of U87 whole cell lysate with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182).Immunohistochemistry
Immunohistochemical staining of human small intestine tissue with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of MCF-7 cells with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182) (diluated at 1:34). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cells with CD9 recombinant monoclonal antibody, clone 3A2 (Cat # RAB04182) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — CD9
Entrez GeneID
928Protein Accession#
P21926Gene Name
CD9
Gene Alias
5H9, BA2, BTCC-1, DRAP-27, GIG2, MIC3, MRP-1, P24, TSPAN29
Gene Description
CD9 molecule
Omim ID
143030Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. [provided by RefSeq
Other Designations
5H9 antigen|CD9 antigen|CD9 antigen (p24)|OTTHUMP00000041574|OTTHUMP00000041576|antigen defined by monoclonal antibody 602-29|growth-inhibiting gene 2 protein|leukocyte antigen MIC3|motility related protein|motility related protein-1|p24 antigen
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Interactome
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Pathway
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Disease
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