HSPD1 recombinant monoclonal antibody, clone 3D8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human and mouse HSPD1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human HSPD1.
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Immunoprecipitation(1:200-1:1000)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of (1) Hela whole cell lysate, (2) HepG2 whole cell lysate, (3) 293 whole cell lysate, (4) Jurkat whole cell lysate, (5) MCF-7 whole cell lysate, (6) K562 whole cell lysate, (7) Mouse brain tissue.Immunohistochemistry
Immunohistochemistry staining in paraffin-embedded human liver tissue.Immunoprecipitation
Immunoprecipitating HSPD1 in HepG2 whole cell lysate.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPD1
Entrez GeneID
3329Protein Accession#
P10809Gene Name
HSPD1
Gene Alias
CPN60, GROEL, HLD4, HSP60, HSP65, HuCHA60, SPG13
Gene Description
heat shock 60kDa protein 1 (chaperonin)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq
Other Designations
P60 lymphocyte protein|chaperonin|heat shock 60kD protein 1 (chaperonin)|heat shock protein 65|mitochondrial heat shock 60kD protein 1 variant 1|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1|spastic paraplegia 13 (autosomal dominant)
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Interactome
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Pathway
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Disease
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