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Western blot analysis of human skeletal muscle tissue with FEN1 recombinant monoclonal antibody, clone SAIC-21C-4 (Cat # RAB03447).
Western Blot analysis the tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of RAB03471 at 0.003 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescent staining of MCF-7 cells with FOXP1 recombinant monoclonal antibody, clone RAB-S37 (Cat # RAB03471)
Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells, permeabilized with 0.15% Triton stained with the chimeric r version of RAB03471 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03471
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow cytometric analysis of MCF-7 cells with the FOXP1 recombinant monoclonal antibody, clone RAB-S37 (Cat # RAB03471).
MCF-7 permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03471 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.