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Western blot analysis of human colon tissue with FEN1 recombinant monoclonal antibody, clone SAIC-21C-4 (Cat # RAB03447). Western Blot analysis the tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of RAB03447 at 1 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescent staining of Hela cells with FEN1 recombinant monoclonal antibody, clone SAIC-21C-4 (Cat # RAB03447).
Immunofluorescence analysis of paraformaldehyde fixed Hela cells, permeabilized with 0.15% Triton stained with the chimeric r version of RAB03447 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1500, showing nucleoli and nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03447
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow cytometric analysis of Hela cells with the FEN1 recombinant monoclonal antibody, clone SAIC-21C-4 (Cat # RAB03447).
Hela cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03447 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.