Products

Western Blot

Western blot analysis of Lane 1: human testis and Lane 2: human ovary lysates with FAS recombinant monoclonal antibody, clone R-125224 (Cat # RAB03438).
Western Blot analysis the lysates (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric r version of RAB03438 at 2 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescence

Immunofluorescent staining of MCF-7 cells with FAS recombinant monoclonal antibody, clone R-125224 (Cat # RAB03438)
Immunofluorescence analysis of paraformaldehyde fixed MCF-7 cells, permeabilized with 0.15% Triton stained with the chimeric mouse IgG1 version of RAB03438 at 10 ug/mL for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-unknown specificity followed by staining with Alexa Fluor® 488 secondary antibody.

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of Jurkat cells with FAS recombinant monoclonal antibody, clone R-125224 (Cat # RAB03438).
Jurkat cells fixed using 2% PFA and permeabilized with 0.5% Triton were stained with unimmunized r antibody (isotype control-black line) or the r chimeric version of RAB03438 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Entrez GeneID

355

Gene Name

FAS

Gene Alias

ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6

Gene Description

Fas (TNF receptor superfamily, member 6)

Omim ID

134637 601859

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq

Other Designations

APO-1 cell surface antigen|CD95 antigen|Fas AMA|Fas antigen|OTTHUMP00000020045|OTTHUMP00000020046|OTTHUMP00000020051|OTTHUMP00000059646|apoptosis antigen 1|tumor necrosis factor receptor superfamily member 6|tumor necrosis factor receptor superfamily, mem