Ptprc recombinant monoclonal antibody, clone RA3-6B2
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Ptprc recombinant monoclonal antibody, clone RA3-6B2 (Cat # RAB03287).
Mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RAB03287 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Ptprc recombinant monoclonal antibody, clone RA3-6B2 (Cat # RAB03287).
Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of RAB03287 (blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against mouse Ptprc.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a popliteal lymph node.
Reactivity
Mouse
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Flow Cytometry
Immunofluorescence
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% Proclin 300)
Storage Instruction
Store at 4°C for 3 months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescent staining of mouse splenocytes with Ptprc recombinant monoclonal antibody, clone RA3-6B2 (Cat # RAB03287).
Mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of RAB03287 at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.Flow Cytometry
Flow cytometric analysis of mouse splenocytes with Ptprc recombinant monoclonal antibody, clone RA3-6B2 (Cat # RAB03287).
Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of RAB03287 (blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer. -
Gene Info — Ptprc
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com