CD34 recombinant monoclonal antibody, clone My10
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More Files
- More Functions
Western Blot
Western blot analysis of Jurkat cell lysate with CD34 recombinant monoclonal antibody, clone My10 (Cat # RAB03229).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta tissue with CD34 recombinant monoclonal antibody, clone My10 (Cat # RAB03229).
Anti-CD34 staining of paraffin embedded human placenta tissue using the rmeric version of RAB03229.RAB03229 was used to stain samples at a concentration of 2-4 ug/ml.
Flow Cytometry
Flow cytometric analysis of human leukocytes with CD34 recombinant monoclonal antibody, clone My10 (Cat # RAB03229).
Human leukocytes were stained with an isotype control (3.0- panel A) or the rmeric version of RAB03229 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD34.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against KG-1a human myeloblastic leukemic cell line.
Reactivity
Human
Form
Liquid
Isotype
IgG
Recommend Usage
Flow Cytometry
Immunohistochemistry
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of Jurkat cell lysate with CD34 recombinant monoclonal antibody, clone My10 (Cat # RAB03229).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human placenta tissue with CD34 recombinant monoclonal antibody, clone My10 (Cat # RAB03229).
Anti-CD34 staining of paraffin embedded human placenta tissue using the rmeric version of RAB03229.RAB03229 was used to stain samples at a concentration of 2-4 ug/ml.Immunoprecipitation
Flow Cytometry
Flow cytometric analysis of human leukocytes with CD34 recombinant monoclonal antibody, clone My10 (Cat # RAB03229).
Human leukocytes were stained with an isotype control (3.0- panel A) or the rmeric version of RAB03229 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody ( and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — CD34
Entrez GeneID
947Gene Name
CD34
Gene Alias
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Gene Description
CD34 molecule
Omim ID
142230Gene Ontology
HyperlinkGene Summary
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM
Other Designations
CD34 antigen|OTTHUMP00000034733|OTTHUMP00000034734|hematopoietic progenitor cell antigen CD34
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Interactome
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Pathway
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Disease
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