PTPN6 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against PTPN6.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant PTPN6.
Theoretical MW (kDa)
68
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of SHP1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:500)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Raji whole cell lysate of Mouse with PTPN6 recombinant monoclonal antibody (Cat # RAB02687) at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded rat brain tissue using PTPN6 recombinant monoclonal antibody (Cat # RAB02687). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using PTPN6 recombinant monoclonal antibody (Cat # RAB02687)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
Flow cytometric analysis of Hela cells with PTPN6 recombinant monoclonal antibody (Cat # RAB02687) at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. -
Gene Info — PTPN6
Entrez GeneID
5777Protein Accession#
P29350Gene Name
PTPN6
Gene Alias
HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1
Gene Description
protein tyrosine phosphatase, non-receptor type 6
Omim ID
176883Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq
Other Designations
hematopoietic cell phosphatase|hematopoietic cell protein-tyrosine phosphatase|protein-tyrosine phosphatase 1C
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