BAD recombinant monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:MCF7 whole cell lysate with BAD recombinant monoclonal antibody (Cat # RAB02676) at 1:500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD recombinant monoclonal antibody (Cat # RAB02676). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using BAD recombinant monoclonal antibody (Cat # RAB02676)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against BAD.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant BAD.
Theoretical MW (kDa)
24
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Bad and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:20-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:MCF7 whole cell lysate with BAD recombinant monoclonal antibody (Cat # RAB02676) at 1:500 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD recombinant monoclonal antibody (Cat # RAB02676). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using BAD recombinant monoclonal antibody (Cat # RAB02676)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
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Gene Info — BAD
Entrez GeneID
572Protein Accession#
Q92934Gene Name
BAD
Gene Alias
BBC2, BCL2L8
Gene Description
BCL2-associated agonist of cell death
Omim ID
603167Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq
Other Designations
BCL-X/BCL-2 binding protein|BCL2-antagonist of cell death protein|BCL2-binding component 6|BCL2-binding protein
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Interactome
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Pathway
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Disease
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