CCND1 recombinant monoclonal antibody
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against CCND1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant CCND1.
Theoretical MW (kDa)
36
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Cyclin D1 and does not cross-react with related proteins.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS , pH7.2 (0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:Hela whole cell lysate Lane2:PC12 whole cell lysate Lane3:SH-SY-5Y whole cell lysate with CCND1 recombinant monoclonal antibody (Cat # RAB02671) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using CCND1 recombinant monoclonal antibody (Cat # RAB02671). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of N2A cells using CCND1 recombinant monoclonal antibody (Cat # RAB02671)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Immunoprecipitation
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Gene Info — CCND1
Entrez GeneID
595Protein Accession#
P24385Gene Name
CCND1
Gene Alias
BCL1, D11S287E, PRAD1, U21B31
Gene Description
cyclin D1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq
Other Designations
B-cell CLL/lymphoma 1|G1/S-specific cyclin D1
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Interactome
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Pathway
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Disease
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