Products

Western Blot (Cell lysate)

Western blot analysis of Jurkat (A) whole cell lysates with BIRC5 recombinant monoclonal antibody (Cat # RAB02542).

Immunocytochemistry

Immunofluorescence

Immunofluorescent analysis of MCF7 cells with BIRC5 recombinant monoclonal antibody (Cat # RAB02542). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Immunoprecipitation

Entrez GeneID

332

Protein Accession#

O15392

Gene Name

BIRC5

Gene Alias

API4, EPR-1

Gene Description

baculoviral IAP repeat-containing 5

Omim ID

603352

Gene Ontology

Hyperlink

Gene Summary

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq

Other Designations

apoptosis inhibitor 4|baculoviral IAP repeat-containing protein 5|survivin variant 3 alpha