HSPA1A recombinant monoclonal antibody, clone R01-6H6
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HSPA1A.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human HSPA1A.
Theoretical MW (kDa)
Calculated MW: 70 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: K562, Lane 2: rat brain, Lane 3: C6 and Lane 4: 3T3 lysates with HSPA1A recombinant monoclonal antibody, clone R01-6H6 (Cat # RAB02195).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human tonsil with HSPA1A recombinant monoclonal antibody, clone R01-6H6 (Cat # RAB02195). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — HSPA1A
Entrez GeneID
3303Protein Accession#
P0DMV8Gene Name
HSPA1A
Gene Alias
FLJ54303, FLJ54370, FLJ54392, FLJ54408, FLJ75127, HSP70-1, HSP70-1A, HSP70I, HSP72, HSPA1, HSPA1B
Gene Description
heat shock 70kDa protein 1A
Omim ID
140550Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq
Other Designations
OTTHUMP00000029287|dnaK-type molecular chaperone HSP70-1|heat shock 70kD protein 1A|heat shock 70kDa protein 1B|heat shock-induced protein
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Interactome
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