MDM2 recombinant monoclonal antibody, clone R08-8C7
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2
Theoretical MW (kDa)
Calculated MW: 55 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1,000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Phospho-MDM2 (Ser166) in Jurkat, C6, Hela lysates using human Phospho-MDM2 (Ser166) recombinant monoclonal antibody, clone R08-8C7 (Cat # RAB01668).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer with Phospho-MDM2 (Ser166) recombinant monoclonal antibody, clone R08-8C7 (Cat # RAB01668). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. -
Gene Info — MDM2
Entrez GeneID
4193Protein Accession#
Q00987Gene Name
MDM2
Gene Alias
HDMX, MGC71221, hdm2
Gene Description
Mdm2 p53 binding protein homolog (mouse)
Omim ID
164785Gene Ontology
HyperlinkGene Summary
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq
Other Designations
Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|mouse double minute 2 homolog|p53-binding protein MDM2|ubiquitin-protein ligase E3 Mdm2
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Interactome
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Pathway
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Disease
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