CD33 recombinant monoclonal antibody, clone hP67.6 (Gemtuzumab)
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
ELISA Plate coated with human His-tagged CD33 at a concentration of 2 ug/mL. A 3-fold serial dilution from 10,000 to 0.1 ng/mL was performed using. For detection, a 1:4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.
Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human CD33.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against by huminization of the murine P67.6 clone, raised against CD33-transfected FMY9S5 cells of human origin. CD33 is a transmembrane receptor expressed on predominantly on cells of the myeloid lineage, but it can also be found on some lymphoid cells.
Reactivity
Human
Specificity
Recognises human CD33.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG, Kappa
Recommend Usage
ELISA
Immunofluorescence
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Enzyme-linked Immunoabsorbent Assay
ELISA Plate coated with human His-tagged CD33 at a concentration of 2 ug/mL. A 3-fold serial dilution from 10,000 to 0.1 ng/mL was performed using. For detection, a 1:4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — CD33
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Interactome
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Pathway
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Disease
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