PLIN1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PLIN1.
Immunogen
A synthetic peptide corresponding to human PLIN1.
Sequence
C-EPILGRAQYSQLRKK
Host
Goat
Theoretical MW (kDa)
56
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.01-0.03 ug/mL)
Immunofluorescence (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PLIN1 polyclonal antibody (Cat # PAB7250) (0.01 ug/mL) staining of human adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunofluorescence
PLIN polyclonal antibody (Cat # PAB7250) staining of differentiated 3T3-L1 adipocytes. Data kindly provided by Prof. J. Granneman, Detroit, USAEnzyme-linked Immunoabsorbent Assay
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Gene Info — PLIN
Entrez GeneID
5346Protein Accession#
NP_002657.2Gene Name
PLIN
Gene Alias
PERI
Gene Description
perilipin
Omim ID
170290Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
lipid droplet-associated protein
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Interactome
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Pathway
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Disease
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Publication Reference
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A proposed model of fat packaging by exchangeable lipid droplet proteins.
Wolins NE, Brasaemle DL, Bickel PE.
FEBS Letters 2006 Sep; 580(23):5484.
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A proposed model of fat packaging by exchangeable lipid droplet proteins.
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