FHL2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
FHL2 polyclonal antibody (Cat # PAB6596) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FHL2.
Immunogen
A synthetic peptide corresponding to human FHL2.
Sequence
RDDILCPDCGKDI
Host
Goat
Theoretical MW (kDa)
32.2
Reactivity
Human, Mouse
Specificity
NP_001441.4, NP_963849.1, NP_963851.2 and NP_0010.34581.1 represent varients of the same protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FHL2 polyclonal antibody (Cat # PAB6596) (1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FHL2
Entrez GeneID
2274Protein Accession#
NP_001441.4;NP_963849.1;NP_963851.2;NP_0010.34581.1Gene Name
FHL2
Gene Alias
AAG11, DRAL, SLIM3
Gene Description
four and a half LIM domains 2
Omim ID
602633Gene Ontology
HyperlinkGene Summary
LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM
Other Designations
aging-associated gene 11|down-regulated in rhabdomyosarcoma LIM protein|four and a half LIM-domain protein 2
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Interactome
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Publication Reference
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Slim defines a novel family of LIM-proteins expressed in skeletal muscle.
Morgan MJ, Madgwick AJ.
Biochemical and Biophysical Research Communications 1996 Aug; 225(2):632.
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Slim defines a novel family of LIM-proteins expressed in skeletal muscle.
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