POU2AF1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of POU2AF1.
Immunogen
A synthetic peptide corresponding to human POU2AF1.
Sequence
C-DAYALNHTLSVEGF
Host
Goat
Theoretical MW (kDa)
27.4
Reactivity
Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
POU2AF1 polyclonal antibody (Cat # PAB6265) staining (2 ug/mL) of mouse spleen lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — POU2AF1
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Interactome
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Disease
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Publication Reference
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OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.
Strubin M, Newell JW, Matthias P.
Cell 1995 Feb; 80(3):497.
Application:WB-Ce, WB-Tr, Human, Mammalian cells.
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OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins.
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