ADK polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ADK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human ADK.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of ADK polyclonal antibody (Cat # PAB4654) in mouse liver tissue lysate (35 ug/lane). ADK (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with ADK polyclonal antibody (Cat # PAB4654), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADK
Entrez GeneID
132Protein Accession#
NP_006712Gene Name
ADK
Gene Alias
AK
Gene Description
adenosine kinase
Omim ID
102750Gene Ontology
HyperlinkGene Summary
This gene encodes adenosine kinase, an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity. [provided by RefSeq
Other Designations
OTTHUMP00000019864|OTTHUMP00000019865|adenosine 5'-phosphotransferase
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Interactome
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Publication Reference
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Coupling of CFTR-mediated anion secretion to nucleoside transporters and adenosine homeostasis in Calu-3 cells.
Szkotak AJ, Ng AM, Man SF, Baldwin SA, Cass CE, Young JD, Duszyk M.
The Journal of Membrane Biology 2003 Apr; 192(3):169.
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Gene structure for adenosine kinase in Chinese hamster and human: high-frequency mutants of CHO cells involve deletions of several introns and exons.
Singh B, Lin A, Wu ZC, Gupta RS.
DNA and Cell Biology 2001 Jan; 20(1):53.
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Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein.
Van Rompay AR, Johansson M, Karlsson A.
European Journal of Biochemistry 1999 Apr; 261(2):509.
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Coupling of CFTR-mediated anion secretion to nucleoside transporters and adenosine homeostasis in Calu-3 cells.
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