DNMT1 (phospho S714) polyclonal antibody
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More Files
- More Functions
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with DNMT1 (phospho S714) polyclonal antibody (Cat # PAB29592) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human DNMT1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S714 of human DNMT1.
Host
Rabbit
Theoretical MW (kDa)
200
Reactivity
Human
Specificity
DNMT1 (phospho S714) polyclonal antibody detects endogenous levels of human DNMT1 only when phosphorylated at serine 714.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with DNMT1 (phospho S714) polyclonal antibody (Cat # PAB29592) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining. -
Gene Info — DNMT1
Entrez GeneID
1786Protein Accession#
P26358Gene Name
DNMT1
Gene Alias
AIM, CXXC9, DNMT, FLJ16293, MCMT, MGC104992
Gene Description
DNA (cytosine-5-)-methyltransferase 1
Omim ID
126375Gene Ontology
HyperlinkGene Summary
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CXXC finger protein 9|DNA methyltransferase 1
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Interactome
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Pathway
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Disease
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