EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of Lane 1: serum treated K562 cells, Lane 2: antigen-specific peptide treated K562 cells with EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody (Cat # PAB29232) at 1:500-1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody (Cat # PAB29232) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human EFNB1/EFNB2/EFNB3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y324 of human EFNB1/EFNB2/EFNB3.
Host
Rabbit
Theoretical MW (kDa)
46
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of EFNB1/EFNB2/EFNB3 only when phosphorylated at tyrosine 324.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (without Mg2+ and Ca2+), (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: serum treated K562 cells, Lane 2: antigen-specific peptide treated K562 cells with EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody (Cat # PAB29232) at 1:500-1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human brain tissue by EFNB1/EFNB2/EFNB3 (phospho Y324) polyclonal antibody (Cat # PAB29232) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — EFNB1
Entrez GeneID
1947Protein Accession#
P98172;P52799;Q15768Gene Name
EFNB1
Gene Alias
CFND, CFNS, EFL3, EPLG2, Elk-L, LERK2, MGC8782
Gene Description
ephrin-B1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq
Other Designations
ELK ligand|OTTHUMP00000023456|eph-related receptor tyrosine kinase ligand 2|ligand of eph-related kinase 2
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Gene Info — EFNB2
Entrez GeneID
1948Protein Accession#
P98172;P52799;Q15768Gene Name
EFNB2
Gene Alias
EPLG5, HTKL, Htk-L, LERK5, MGC126226, MGC126227, MGC126228
Gene Description
ephrin-B2
Omim ID
600527Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq
Other Designations
HTK ligand|eph-related receptor tyrosine kinase ligand 5|ephrin B2|ligand of eph-related kinase 5
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Gene Info — EFNB3
Entrez GeneID
1949Protein Accession#
P98172;P52799;Q15768Gene Name
EFNB3
Gene Alias
EFL6, EPLG8, LERK8
Gene Description
ephrin-B3
Omim ID
602297Gene Ontology
HyperlinkGene Summary
EFNB3, a member of the ephrin gene family, is important in brain development as well as in its maintenance. Moreover, since levels of EFNB3 expression were particularly high in several forebrain subregions compared to other brain subregions, it may play a pivotal role in forebrain function. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH Receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq
Other Designations
Ephrin B3|eph-related receptor tyrosine kinase ligand 8
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