MMP3 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MMP3.
Immunogen
A synthetic peptide corresponding to amino acids 459-473 at C-terminus of human MMP3.
Sequence
DPNAKKVTHTLKSNS
Host
Goat
Theoretical MW (kDa)
52
Reactivity
Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Immunohistochemistry (4-6 ug/mL)
Western Blot (0.1-0.3ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MMP3 polycolnal antibody (Cat # PAB27568) (0.2ug/ml) staining of prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Cell lysate)
MMP3 polycolnal antibody (Cat # PAB27568) (0.3 ug/mL) staining of Mouse Eye lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
MMP3 polycolnal antibody (Cat # PAB27568) (6 ug/mL) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MMP3
Entrez GeneID
4314Protein Accession#
NP_002413.1Gene Name
MMP3
Gene Alias
CHDS6, MGC126102, MGC126103, MGC126104, MMP-3, SL-1, STMY, STMY1, STR1
Gene Description
matrix metallopeptidase 3 (stromelysin 1, progelatinase)
Omim ID
185250Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq
Other Designations
matrix metalloproteinase 3|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|progelatinase|proteoglycanase|stromelysin 1|transin-1
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Interactome
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Disease
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