Grm1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of 10 ug of HEK 293 cells expressing mGluR5 (Lane 1) and Grm1 (Lane 2) showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of Grm1. The Grm1 polyclonal antibody (Cat # PAB14526) shows no reactivity toward mGluR5.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Grm1.
Immunogen
A synthetic peptide corresponding to C-terminus rat Grm1.
Host
Rabbit
Theoretical MW (kDa)
125, 250
Reactivity
Rat
Specificity
Specific for the ~125k monomer and the ~250k Grm1 dimer.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (Frozen sections) (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of 10 ug of HEK 293 cells expressing mGluR5 (Lane 1) and Grm1 (Lane 2) showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of Grm1. The Grm1 polyclonal antibody (Cat # PAB14526) shows no reactivity toward mGluR5.Immunohistochemistry (Frozen sections)
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Gene Info — Grm1
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Publication Reference
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Anatomical and molecular characterization of dopamine D1 receptor-expressing neurons of the mouse CA1 dorsal hippocampus.
Puighermanal E, Cutando L, Boubaker-Vitre J, Honoré E, Longueville S, Hervé D, Valjent E.
Brain Structure & Function 2016 Sep; [Epub].
Application：IF, Mouse, Mouse dorsal hippocampus.
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Drd2-Cre:RiboTag mouse line unravels the possible diversity of dopamine D2 receptor-expressing cells of the dorsal mouse hippocampus.
Puighermanal E, Biever A, Espallergues J, Gangarossa G, De Bundel D, Valjent E.
Hippocampus 2015 Jul; 25(7):858.
Application：IS, Mouse, Dorsal hippocampus.
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Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis.
Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SS.
The Journal of Neuroscience 2004 Oct; 24(40):8752.
Application：IP, WB-Tr, Human, HEK 293 cells.
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Neuroprotection against NMDA excitotoxicity by group I metabotropic glutamate receptors is associated with reduction of NMDA stimulated currents.
Blaabjerg M, Fang L, Zimmer J, Baskys A.
Experimental Neurology 2003 Oct; 183(2):573.
Application：Added, Recombinant protein.
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Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses.
Wilson RI, Nicoll RA.
Nature 2001 Mar; 410(6828):588.
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Anatomical and molecular characterization of dopamine D1 receptor-expressing neurons of the mouse CA1 dorsal hippocampus.
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