TP53 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TP53.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human TP53.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:100-1:1000)
ChIP (2 ug/ChIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using the U-2 OS (human osteosarcoma cell line), TP53 polyclonal antibody (Cat # PAB14128) and optimized PCR primer sets for PCR.
Chromatin sheared from 1x106 cells and 2 ug of antibody against TP53, or beads only were used per ChIP experiment.
Figure shows the recovery expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Upper : recovery by TP53 of the TP21 promoter, a known target for TP53 (1), or beads only.
Recovery of the TP21 promoter by the TP53 antibody is evident based on fluorescent qPCR analysis of immunoprecipitated DNA.
Right : recovery of the GAPDH promoter (used as a negative control) by TP53 or beads only.Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of TP53 polyclonal antibody (Cat # PAB14128), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 4,000. -
Gene Info — TP53
Entrez GeneID
7157Protein Accession#
P04637Gene Name
TP53
Gene Alias
FLJ92943, LFS1, TRP53, p53
Gene Description
tumor protein p53
Gene Ontology
HyperlinkGene Summary
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq
Other Designations
p53 antigen|p53 transformation suppressor|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53
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Interactome
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Pathway
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Disease
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Publication Reference
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Requirement for p53 and p21 to sustain G2 arrest after DNA damage.
Bunz F, Dutriaux A, Lengauer C, Waldman T, Zhou S, Brown JP, Sedivy JM, Kinzler KW, Vogelstein B.
Science 1998 Nov; 282(5393):1497.
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Requirement for p53 and p21 to sustain G2 arrest after DNA damage.
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