ABL1/ABL2 (phospho Y393/Y429) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1/ABL2.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y393/Y429 of human ABL1/ABL2.
Sequence
DTYpTA
Host
Rabbit
Theoretical MW (kDa)
210
Reactivity
Human, Mouse
Specificity
ABL1/2 (phospho-Tyr393/429) Antibody detects endogenous levels of ABL1/2 only when phosphorylated at tyrosine393/429.
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190).Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). -
Gene Info — ABL1
Entrez GeneID
25Protein Accession#
P00519 (Gene ID : 25);P42684 (Gene ID : 27)Gene Name
ABL1
Gene Alias
ABL, JTK7, bcr/abl, c-ABL, p150, v-abl
Gene Description
c-abl oncogene 1, receptor tyrosine kinase
Omim ID
189980Gene Ontology
HyperlinkGene Summary
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
Other Designations
Abelson murine leukemia viral (v-abl) oncogene homolog 1|OTTHUMP00000022375|OTTHUMP00000022376|bcr/c-abl oncogene protein|proto-oncogene tyrosine-protein kinase ABL1|v-abl Abelson murine leukemia viral oncogene homolog 1
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Gene Info — ABL2
Entrez GeneID
27Protein Accession#
P00519 (Gene ID : 25);P42684 (Gene ID : 27)Gene Name
ABL2
Gene Alias
ABLL, ARG, FLJ22224, FLJ31718, FLJ41441
Gene Description
v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene)
Omim ID
164690Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinase. The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ETV6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq
Other Designations
Abelson murine leukemia viral (v-abl) oncogene homolog 2|Abelson-related gene protein|OTTHUMP00000033139|arg tyrosine kinase|v-abl Abelson murine leukemia viral oncogene homolog 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Autoinhibition of c-Abl.
Pluk H, Dorey K, Superti-Furga G.
Cell 2002 Jan; 108(2):247.
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c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines.
Brasher BB, Van Etten RA.
The Journal of Biological Chemistry 2000 Nov; 275(45):35631.
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JAK-STAT signaling activated by Abl oncogenes.
Danial NN, Rothman P.
Oncogene 2000 May; 19(21):2523.
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Autoinhibition of c-Abl.
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