SMAD3 (phospho S213) polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of SMAD3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S213 of human SMAD3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SMAD3 (phospho S213) polyclonal antibody (Cat # PAB0610). Western blot analysis of SMAD3 expression in human liver.Western Blot (Cell lysate)
SMAD3 (phospho S213) polyclonal antibody (Cat # PAB0610). Western blot analysis of SMAD3 expression in A-431.Western Blot (Cell lysate)
The SMAD3 (phospho S213) polyclonal antibody (Cat # PAB0610) is used in Western blot to detect Phospho-SMAD3-S213 in Ramos cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SMAD3 (phospho S213) polyclonal antibody (Cat # PAB0610) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — SMAD3
Entrez GeneID
4088Protein Accession#
NP_005893;P84022Gene Name
SMAD3
Gene Alias
DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396
Gene Description
SMAD family member 3
Omim ID
603109Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq
Other Designations
MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|mad homolog JV15-2|mad protein homolog|mothers against decapentaplegic homolog 3
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Interactome
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Pathway
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Disease
- Alzheimer disease
- Anemia
- Asthma
- Bacteremia
- Breast cancer
- Breast Neoplasms
- Cleft Lip
- Cleft Palate
- Coronary Artery Disease
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Publication Reference
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Transforming growth factor-beta1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts.
Jablonska E, Markart P, Zakrzewicz D, Preissner KT, Wygrecka M.
The Journal of Biological Chemistry 2010 Apr; 285(15):11638.
Application:WB, Human, Lung fibroblasts.
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Transforming growth factor-beta1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts.
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