FGF1 (Human) Recombinant Protein
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Specification
Regulatory Status
RUO grade
Product Description
Human FGF1 recombinant protein with His tag at the C-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 16.77
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 98% by SDS-PAGE
Endotoxin Level
< 0.1 EU/ug
Activity
The ED50 for this effect is < 0.3 ng/mL, measured by its ability to induce 3T3 cells proliferation. The specific activity of recombinant human FGF-1 is > 1.0 x 106 IU/mg.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS, pH 8.0.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 200 ug/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — FGF1
Entrez GeneID
2246Gene Name
FGF1
Gene Alias
AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Gene Description
fibroblast growth factor 1 (acidic)
Omim ID
131220Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000066028|OTTHUMP00000066030|OTTHUMP00000066031|OTTHUMP00000174675|endothelial cell growth factor, alpha|endothelial cell growth factor, beta|heparin-binding growth factor 1
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