LRG1 (Human) Recombinant Protein
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Specification
Product Description
Human LRG1 (P02750, Val36-Gln347) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Val36-Gln347
Host
Human
Theoretical MW (kDa)
35.4
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.0 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human LRG1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human LRG1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human LRG1, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 8.0 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — LRG1
Entrez GeneID
116844Protein Accession#
P02750Gene Name
LRG1
Gene Alias
HMFT1766, LRG
Gene Description
leucine-rich alpha-2-glycoprotein 1
Omim ID
611289Gene Ontology
HyperlinkGene Summary
The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation (O'Donnell et al., 2002 [PubMed 12223515]).[supplied by OMIM
Other Designations
1300008B03Rik|2310031E04Rik|leucine rich alpha 2 glycoprotein
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