LGALS1 (Human) Recombinant Protein
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Specification
Product Description
Human LGALS1 (P09382, Ala2-Asp135) partial recombinant protein with hFc tag at N-Terminus expressed in HEK293 cells.
Sequence
Ala2-Asp135
Host
Human
Theoretical MW (kDa)
41.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 25.9 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Galectin 1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Galectin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Galectin 1, hFc Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-Galectin 1 Antibody, hFc Tag with the EC50 of 25.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — LGALS1
Entrez GeneID
3956Protein Accession#
P09382Gene Name
LGALS1
Gene Alias
DKFZp686E23103, GAL1, GBP
Gene Description
lectin, galactoside-binding, soluble, 1
Omim ID
150570Gene Ontology
HyperlinkGene Summary
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq
Other Designations
OTTHUMP00000028982|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|galaptin|galectin 1|galectin-1|lactose-binding lectin 1
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