CCL24 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human CCL24, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CCL24 Antibody, hFc Tag with the EC50 of 16.8 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human CCL24 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CCL24 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CCL24 (O00175, Val27-Cys119) partial recombinant protein with His tag at N-Terminus expressed in HEK293 cells.
Sequence
Val27-Cys119
Host
Human
Theoretical MW (kDa)
11.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 34.4 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CCL24 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CCL24 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CCL24, His Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CCL24 Antibody, hFc Tag with the EC50 of 16.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CCL24
Entrez GeneID
6369Protein Accession#
O00175Gene Name
CCL24
Gene Alias
Ckb-6, MPIF-2, MPIF2, SCYA24
Gene Description
chemokine (C-C motif) ligand 24
Omim ID
602495Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. [provided by RefSeq
Other Designations
CK-beta-6|eotaxin-2|myeloid progenitor inhibitory factor 2|small inducible cytokine A24|small inducible cytokine subfamily A (Cys-Cys), member 24
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Interactome
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Pathway
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Disease
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