AXL (Human) Recombinant Protein
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Specification
Product Description
Human AXL (P30530-1, Ala26-Pro449) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Ala26-Pro449
Host
Human
Theoretical MW (kDa)
72.5999999999998
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.15 ug/mL, messured by ELISA at 2 ug/mL. The affinity constant of 1.57 nM as determined in SPR assay (Biacore T200)
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Biotinylated Human Axl is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Biotinylated Human Axl on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-Axl Antibody at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human Axl, hFc Tag with the EC50 of 26.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — AXL
Entrez GeneID
558Protein Accession#
P30530-1Gene Name
AXL
Gene Alias
JTK11, UFO
Gene Description
AXL receptor tyrosine kinase
Omim ID
109135Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
AXL transforming sequence/gene|oncogene AXL
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Interactome
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Disease
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