TF (Human) Recombinant Protein
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Specification
Product Description
Human TF (AAH59367, Val20-Pro698) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Val20-Pro698
Host
Human
Theoretical MW (kDa)
76.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The affinity constant of 27.50 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Transferrin is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Transferrin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -81°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 27.50 nM as determined in SPR assay (Biacore T200). -
Gene Info — TF
Entrez GeneID
7018Protein Accession#
AAH59367Gene Name
TF
Gene Alias
DKFZp781D0156, PRO1557, PRO2086
Gene Description
transferrin
Gene Ontology
HyperlinkGene Summary
This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq
Other Designations
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Interactome
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Disease
- Abortion
- Alzheimer disease
- Anemia
- Anoxia
- Arthritis
- Asthma
- Atherosclerosis
- Birth Weight
- Brain Ischemia
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