CD7 (Human) Recombinant Protein
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Specification
Product Description
Human CD7 (P09564, 26 a.a. - 180 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cells.
Sequence
AQEVQQSPHCTTVPVGASVNITCSTSGGLRGIYLRQLGPQPQDIIYYEDGVVPTTDRRFRGRIDFSGSQDNLTITMHRLQLSDTGTYTCQAITEVNVYGSGTLVLVTEEQSQGWHRCSDAPPRASALPAPPTGSALPDPQTASALPDPPAASALP
Host
Human
Theoretical MW (kDa)
43.19
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 27.7 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD7 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD7, hFc Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-CD7 Antibody, hFc Tag with the EC50 of 27.7 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CD7
Entrez GeneID
924Protein Accession#
P09564Gene Name
CD7
Gene Alias
GP40, LEU-9, TP41, Tp40
Gene Description
CD7 molecule
Omim ID
186820Gene Ontology
HyperlinkGene Summary
This gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. [provided by RefSeq
Other Designations
CD7 antigen|CD7 antigen (p41)|T-cell antigen CD7|T-cell leukemia antigen|p41 protein
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