Fgf9 (Rat) Recombinant Protein
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Specification
Product Description
Rat Fgf9 recombinant protein expressed in?Escherichia coli.
Sequence
MPLGEVGSYFGVQDAVPFGNVPVLPVDSPVLLNDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS
Host
Escherichia coli
Theoretical MW (kDa)
23.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatography
Purity
> 95% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 0.5 ng/mL, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake), corresponding to a Specific Activity of 2 x 106Units/mg.
Storage Buffer
Protein(1 mg/mL) was lyophilized from a solution containing 10mM NaP, pH 7.5, 75mM Ammonium Sulfate. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — Fgf9
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