IL2 (Human) Recombinant Protein
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Specification
Product Description
Human IL2 recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 16.4
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.05 EU/ug
Activity
The ED50 for this effect is < 3 ng/mL, measured by the induction of proliferation in CTLL-2 cells. The specific activity of recombinant human IL-2 is > 5 x 106 IU/mg, which is calibrated against the human IL-2 WHO International Standard (NIBSC code: 86/500).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Regulatory Status
GMP grade
Storage Buffer
Lyophilized from NaPi buffer, 0.018% SDS, pH 7.5.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is <0.2 ng/mL, measured by the induction of proliferation in CTLL-2 cells.SDS-PAGE
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Gene Info — IL2
Entrez GeneID
3558Gene Name
IL2
Gene Alias
IL-2, TCGF, lymphokine
Gene Description
interleukin 2
Omim ID
147680Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq
Other Designations
T cell growth factor|aldesleukin|interleukin-2|involved in regulation of T-cell clonal expansion
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