Il33 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Il33 (NP_001158196.1, 109 - 266 a.a.) partial length recombinant protein with His tag expressed in Escherichia coli expression system.
Host
Escherichia coli
Theoretical MW (kDa)
18.1
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Mouse Il33 stimulates cell proliferation D10.G4.1 mouse helper T cells. The ED50 range ≤ 0.1 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
3 ug SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 1 mM DTT, pH 7.4 (10% glycerol)
Storage Instruction
Store at 4°C for one week. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — Il33
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