ALPP (Human) Recombinant Protein
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Specification
Product Description
Human ALPP (AAH09647.1, 23 a.a. - 506 a.a.) partial recombinant protein with His tag expressed in Baculovirus expression system.
Host
Viruses
Theoretical MW (kDa)
53.9
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU/ug
Activity
Specific activity is > 2,500 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
SDS-PAGE Stained with Coomassie Blue.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — ALPP
Entrez GeneID
250Protein Accession#
P05187Gene Name
ALPP
Gene Alias
ALP, FLJ61142, PALP, PLAP
Gene Description
alkaline phosphatase, placental (Regan isozyme)
Omim ID
171800Gene Ontology
HyperlinkGene Summary
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq
Other Designations
alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase
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