PGD (Human) Recombinant Protein
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Specification
Product Description
Human PGD (NP_002622, 1 a.a. - 483 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
55.3
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 90% by SDS-PAGE
Activity
Specific activity is > 10 unit/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25°C, in the presence of beta-NADP.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
15% SDS-PAGE Stained with Coomassie Blue.
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 1 mM DTT, 0.1 M NaCl, pH 8.0 (10% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
SDS-PAGE
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Gene Info — PGD
Entrez GeneID
5226Protein Accession#
P52209Gene Name
PGD
Gene Alias
6PGD
Gene Description
phosphogluconate dehydrogenase
Omim ID
172200Gene Ontology
HyperlinkGene Summary
6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq
Other Designations
6-phosphogluconate dehydrogenase
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Interactome
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