S (SARS-CoV-2) RBD Recombinant Protein
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Specification
Product Description
SARS-CoV-2 S RBD (YP_009724390.1, 319 a.a. - 541 a.a.) recombinant protein with mFc tag at C-terminal expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
50.8
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Affinity purification
Purity
> 95% by SDS-PAGE.
Endotoxin Level
< 1.0 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized Recombinant human ACE2 at 2 ug/mL (100 uL/well) can bind S (SARS-CoV-2) RBD Recombinant Protein, the EC50 of S (SARS-CoV-2) RBD Recombinant Protein is 2.58 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60 kDa.
Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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