S1 (SARS-CoV-2) Recombinant Protein
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Specification
Product Description
SARS-CoV-2 S1 (YP_009724390.1, 11 a.a. - 682 a.a.) recombinant protein with Fc-His tag at C-terminal expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
103
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Affinity purification
Purity
> 95% by SDS-PAGE.
Endotoxin Level
< 1.0 EU/ug of the protein by LAL method.
Activity
A. Measured by the binding ability in a functional ELISA. Immobilized recombinant human ACE2 at 2 ug/mL (100 uL/well) can bind S1 (SARS-CoV-2) Recombinant Protein with a linear range of 78-82.5 ng/mL. B. Immobilized human ACE2 on COOH Chip, can bind this protein with an affinity constant of 90.8 nM as determined in a SPR assay (Nicoya OpenSPR).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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