MAPK15 (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human MAPK15 (NP_620590.2, 1 a.a. - 544 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
87
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
94 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Histone H1 peptide. ATP: 500 uM
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MAPK15
Entrez GeneID
225689Protein Accession#
NP_620590.2Gene Name
MAPK15
Gene Alias
ERK7, ERK8
Gene Description
mitogen-activated protein kinase 15
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000189334|extracellular regulated kinase 8 delta|extracellular signal regulated kinase 8|extracellular signal-regulated kinase 8
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