CDK6/CCND3 (Human) Recombinant Protein
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Specification
Product Description
Human CDK6 (NP_001250.1, 1 a.a. - 326 a.a.) and CCND3 (AAA51927.1, 1 a.a. - 292 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
64
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
94 % by SDS-PAGE/CBB staining
Activity
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: DYRKtide-F. ATP: 200 uM
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CCND3
Entrez GeneID
896Protein Accession#
NP_001250.1 (Gene ID : 1021);AAA51927.1 (Gene ID : 896)Gene Name
CCND3
Gene Alias
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Gene Description
cyclin D3
Omim ID
123834Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
D3-type cyclin|G1/S-specific cyclin D3|OTTHUMP00000016390
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Gene Info — CDK6
Entrez GeneID
1021Protein Accession#
NP_001250.1 (Gene ID : 1021);AAA51927.1 (Gene ID : 896)Gene Name
CDK6
Gene Alias
MGC59692, PLSTIRE, STQTL11
Gene Description
cyclin-dependent kinase 6
Omim ID
603368Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. [provided by RefSeq
Other Designations
cell division protein kinase 6
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Interactome
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Pathway
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Disease
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